TrueTag TechnologyThe ParAllele approach to comprehensive genomic assays achieves its unprecedented specificity and sensitivity by separating the highly multiplexed detection of complex genome sequences and the highly multiplexed detection of assay products. This is achieved by harnessing the specificity of multiple enzymes for detection at the genomic level and the specificity of engineered molecular tags for detection of complex assay products. The latter is TrueTag technology.
ParAllele has developed a proprietary set of 21-mer oligonucleotide tags (TrueTag) used for detection of complex genomic assays on beads or arrays. These tags have been designed and tested to have highly similar performance in PCR and array hybridization, which results in robust and reproducible signals on multiple microarray- and bead-based platforms. The tags are also designed to be maximally different from each other in terms of sequence. The set of ParAllele TrueTags was computed using sophisticated algorithms, including maximizing the mismatch between any pair in the set, starting from the possible four trillion 21-mer DNA sequences. Because of these stringent criteria, this TrueTag universal set has been measured to have minimal cross-hybridization properties compared to other such sets of universal tags. This makes the TrueTag an ideal set of sequences for separation by hybridization to solid-support-oligonucleotide detection platforms such as microarrays and beads.
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